含有死海礦物質的乳霜對UVB引起的人體皮膚壓力的保護作用

美國國家醫學圖書館 國立衛生研究院   https://www.ncbi.nlm.nih.gov

 2009 Sep;18(9):781-8. doi: 10.1111/j.1600-0625.2009.00865.x. Epub 2009 Mar 23.

耶路撒冷希伯來大學藥學院藥劑學系 Department of Pharmaceutics, School of Pharmacy, Hebrew University of Jerusalem, Jerusalem, Israel.

Protective effects of a cream containing Dead Sea minerals against UVB-induced stress in human skin

含有死海礦物質的乳霜對UVB引起的人體皮膚壓力的保護作用

 

Abstract 節錄

Dead Sea (DS) mud and water are known for their unique composition of minerals, and for their therapeutic properties on psoriasis and other inflammatory skin diseases. 

死海(DS)泥和水以其獨特的礦物質成分以及對牛皮癬和其他炎症性皮膚病的治療特性而聞名。

 

Their mode of action, however, remains poorly known. To analyse the ability of - a leave-on skin preparation containing DS mud and other ingredients like DS water, zinc oxide, aloe-vera extract, pro-vitamin B5 and vitamin E, to antagonize biological effects induced by UVB irradiation in skin when topically applied in organ cultures. 

然而,他們的作用模式仍然鮮為人知。 研究分析(一種含有死海泥和其他成分,如死海水,氧化鋅,蘆薈提取物,維生素原B5和維生素E),當局部應用在器官培養物中時,可拮抗UVB輻射在皮膚中引起的生物效應。

 

Skin pieces were analysed for mitochondrial activity by MTT assay, for apoptosis by caspase 3 assay, for cytokine secretion by solid phase ELISA, for overall antioxidant capacity by ferric reducing antioxidant power and Oxygen radical absorbance capacity assays (epidermis) or by cyclic voltammetry (external medium), and for uric acid (UA) content by HPLC. We report that UVB irradiation decreases cell viability, total antioxidant capacity and UA contents in the epidermis of skin organ cultures, while increasing the levels of apoptosis in cells and their cytokine secretion. 

我們通過MTT分析法分析皮膚塊的線粒體活性,通過caspase 3分析法分析細胞凋亡,通過固相ELISA分析細胞因子分泌,通過三價鐵還原抗氧化劑能力和氧自由基吸收能力分析法(表皮)或通過循環伏安法(外部培養基),並通過HPLC測定尿酸(UA)的含量。報告指出,UVB輻射降低皮膚器官培養物表皮中的細胞活力,總抗氧化能力和UA含量,同時增加細胞凋亡的等級及其細胞因子的分泌。

 

附註: 氧化壓力(oxidative stress)是指當自由基與抗氧化物比值間不平衡的狀態,尤其是自由基過剩的情況下,抗氧化物被過度耗損的失衡狀態。(網路查得)

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